Home Research Services Publications People Teaching Job Opening News Lab Only
Online Services

I-TASSER QUARK LOMETS COACH COFACTOR MUSTER SEGMER FG-MD ModRefiner REMO SPRING COTH BSpred SVMSEQ ANGLOR BSP-SLIM SAXSTER ThreaDom EvoDesign GPCR-I-TASSER BindProf BindProfX ResQ IonCom STRUM

TM-score TM-align MMalign NWalign EDTSurf MVP MVP-Fit SPICKER HAAD PSSpred 3DRobot I-TASSER-MR

BioLiP E. coli GLASS GPCR-HGmod GPCR-RD GPCR-EXP TM-fold DECOYS POTENTIAL RW HPSF CASP7 CASP8 CASP9 CASP10 CASP11 CASP12



COFACTOR is a structure, sequence, and protein-protein interaction (PPI) based method for biological function annotation of protein molecules. Starting from the 3D structural model, COFACTOR will thread the query through the BioLiP protein funtion database by local and global structure matches to identify functional sites and homologies. Functional insights, including Gene Ontology (GO), Enzyme Commission (EC), and ligand-binding sites, will be derived from the best functional homology templates. For GO, additional insights will be obtained from UniProt-GOA by sequence and sequence-profile alignments and from STRING by protein-protein interaction inferrals. The COFACTOR algorithm (as "I-TASSER_FUNCTION") was ranked as the best method for protein function prediction in the community-wide CASP9 experiments. Questions about the COFACTOR server can be posted at the Service System Discussion Board.

For a given target structure, the output of COFACTOR includes (see an illustrative example):

  • The top 10 closest structures in the PDB identified by TM-align
  • GO terms and the directed acyclic graphs associated with the target protein
  • EC numbers, active sites and the enzyme homolog structures of the target protein
  • Ligand binding site on target protein and the ligand-protein complex structures

[Example prediction] [Help] [Library] [Benchmark dataset] [Forum]
  • Structure of Query Protein (Mandatory, [30,1500] residues in PDB format)
      Copy and paste your structure file here    Sample input

      Or upload the stucture file from your local computer
  • Email: (Mandatory, where results will be sent to)

  • Job ID: (Optional, your given name to this protein)

    Advanced options [?]


  • References:

    yangzhanglabumich.edu | (734) 647-1549 | 100 Washtenaw Avenue, Ann Arbor, MI 48109-2218