(The server completed structure and function predictions for 102328 proteins submitted by 27766 users from 105 countries)
(Template library updated on 2012/05/11. Number of visitors: 732296)

I-TASSER server is an Internet service for protein structure and function predictions. 3D models are built based on multiple-threading alignments by LOMETS and iterative TASSER assembly simulations; function inslights are then derived by matching the predicted models with protein function databases. I-TASSER (as 'Zhang-Server') was ranked as the No 1 server for protein structure prediction in recent CASP7, CASP8 and CASP9 experiments. It was also ranked as the best for function prediction in CASP9. The server is in active development with the goal to provide the most accurate structural and function predictions using state-of-the-art algorithms. The server is only for non-commercial use. Please report any problems and questions at I-TASSER message board and some members will study and answer the questions asap. (>> More about the server ...)

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Copy and paste your sequence here (<1,500 residues, in FASTA format): Or upload the sequence from your local computer: Email: (mandatory, where results will be sent to) Password: (mandatory, please click here if you do not have a password) ID: (optional, your given name of the protein) Option I: Assign additional restraints & templates to guide I-TASSER modeling. Assign contact/distance restraints Atom-atom restraints Explanation Specify template without alignment Type a PDB ID Explanation Specify template without alignment Upload a structure Explanation Specify template with alignment Template & alignment Explanation (Read more explanation on how to add restraints) Assign contact/distance restraints Atom-atom restraints Explanation Specify template without alignment Type a PDB ID Explanation Specify template without alignment Upload a structure Explanation Specify template with alignment Template & alignment Explanation Option II: Exclude some templates from I-TASSER template library. Exclude homologous templates Type a cutoff (e.g. '60%') Explanation Exclude specific template proteins Type a PDB ID Explanation (Please submit a new job only after your old job is completed)

• 2012/04/18: The I-TASSER server had the 1,000,000th protein modelled (Congratulations!). Click here to see the most recent statistics.
• 2011/11/03: The I-TASSER Video was published at the JoVE: Journal of Visualized Experiments.
• 2011/10/15: Visualization was enabled for the top 10 proteins analogous to the I-TASSER models and for the enzyme commission predictions.
• 2011/10/09: The function homology search was extended to the entire PDB library. This helps increase the coverage and accuracy of the structure-based function of the I-TASSER server.
• 2011/08/01: The I-TASSER server had the 20,000th user registered (Congratulations!).

  >> Read more I-TASSER news ...

• Ambrish Roy, Alper Kucukural, Yang Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, vol 5, 725-738 (2010). (download the PDF file).
  
• Ambrish Roy, Dong Xu, Jonathan Poisson, Yang Zhang. A Protocol for Computer-Based Protein Structure and Function Prediction. Journal of Visualized Experiments, vol 57, e3259 (2011). (download the PDF file, or Watch the Vedio).
  
• Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, vol 9, 40 (2008). (download the PDF file).