• Exclude specific template proteins: This option allows users to upload a list of template structures that will be excluded from the I-TASSER template library. As the PDB library is redundant and same protein can exist as multiple entries, I-TASSER server will by default exclude the user-specified templates as well as all templates that have a sequence identity >90% to the specified templates. Users can also specify a different sequence identity cutoff, e.g. 70%, where I-TASSER will exclude all templates with a sequence identity >70% to specified template proteins.

    The format of the file should be "PDBID:ChainID   %Seq.Identity", e.g. 

    1wor:A  70
3mxu:A  80
1zko:B  40
    OR 
    1wor:A
3mxu:A
1zko:B

    In general, excluding templates will decrease the quality of I-TASSER modeling. So this option is only designed for some special purposes.