#I want to coat a 10-mer peptide at ELISA wells for recognizing Antiserum against the native surface antigen of a virus.
1)How can I understand this 10-mer peptide has a similar 3D structure to the native surface protein? Please introduce the appropriate approach or software.
2) I wonder why when I checked the 10 top 3D, GO, and other items in the result sheet, non of them were related to the original native protein that I selected 10 aa from it?
3) I have uploaded the pbd downloaded from I-TASSER (related to a protein of SARS2) with the pbd related to 10-mer peptide ((related to the same protein of SARS2) on a server for comparing 3D structure of them, but the 10-mer sequence was not in the answer sheet of that server. I can see my 10-mer aa in the page of I-TASSER.
Please guide me
Thank you very much
Best wishes