The Zhang Lab Message Boards

Hello, Recently I had submitted a job for modeling which had following amino acid sequence:
E V M P A P M P E P E V K I V E K P V E V I V Y R D R V V Q A P A K W K P N G S V G V E L R T Q G K V E N K G K K A T E E N A R K G W A G K E P N V R L E T K A S V N F T E N Q N L E V R T R Q T H V L T K T D S D K E E S N H K D T Q V R I R H T Y N F G K L G S S K V G F K V A S Q Y L H D D H V D S L R T R A V F D F A D Y I Y S N S L F K T T A L E I G P S Y K Y V W G G N D D R Y Y N A L G L Y A N A E F E L P Y G F G F Q A E F E D A F T Y T S T G K G D G K R D K A K L G H A D F V L S H S L D L Y K E G K H S L A F L N E L E Y E T F W A W D K K D A S M E E W P H V D G H G R V N S E G K N K K W G A Y E L T Y T P K L Q Y N Y Q A T E F V K L Y A A I G G E Y V N R E N N K S T A R Y W R W N P T A W A G M K V T F

Hello,

I submitted a job a while ago. I couldn't open the provided link. I got the following massage:

The requested URL /I-TASSER/output/S33359 was not found on this server.

Please advice me about getting the PDB file.

Thanks,

Weiz

My protein of interest contains two different domains for which structures exist in PDB. I would therefore like to run two different I-TASSER predictions and sort of stitch together the resulting models because I believe that this would increase the predictive value.

My questions are:
1) Is this a useful approach?
2) How is this possible?

with best regards and thank you

Axel

I have a PDB-format file for a template structure (one that is not in PDB) that I want to use to build a homology model. It was suggested that I use I-Tasser. I pasted in the sequence I want to model and uploaded the PDB file. However I-Tasser seems to produce results only for sequences already in PDB. How can I get it to use only the uploaded file?

Thanks!

Hello,

I have a question about how the I-TASSER server uses the template the user specifies. If a template is specified, is this the sole template I-TASSER uses to generate the 5 models? Or does it use this template in addition to other templates found by LOMETS?

Many thanks,

Charles

This forum is intended for questions and discussion regarding the I-TASSER system.
Thanks for posting.