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I-TASSER QUARK LOMETS COACH COFACTOR MetaGO MUSTER CEthreader SEGMER FG-MD ModRefiner REMO DEMO SPRING COTH BSpred ANGLOR BSP-SLIM SAXSTER ThreaDom ThreaDomEx EvoDesign GPCR-I-TASSER BindProf BindProfX SSIPe ResQ IonCom STRUM DAMpred

TM-score TM-align MM-align RNA-align NW-align LS-align EDTSurf MVP MVP-Fit SPICKER HAAD PSSpred 3DRobot MR-REX I-TASSER-MR SVMSEQ NeBcon ResPRE WDL-RF ATPbind DockRMSD DeepMSA

BioLiP E. coli GLASS GPCR-HGmod GPCR-RD GPCR-EXP Tara-3D TM-fold DECOYS POTENTIAL RW/RWplus EvoEF HPSF THE-DB CASP7 CASP8 CASP9 CASP10 CASP11 CASP12 CASP13

DEMO (Domain Enhanced MOdeling) is a method for automated assembly of full-length structural models of multi-domain proteins. Starting from individual domain structures, DEMO first identify quaternary structure templates that have similar component domains by domain-level structural alignments using TM-align. Replica-exchange Monte Carlo simulations are then used to assemble full-length models, as guided by the inter-domain distance profiles collected from the top-ranked quaternary templates. The final models with the lowest energy are selected from Monte Carlo trajectories, followed by atomic-level refinments using fragment-guided molecular dynamics simulations. DEMO can be used to assemble domains from either experimental or predicted models for proteins with both continuous and discontinuous domain architectures.

From May. 10 to Jul. 30, 2020 (CASP14 season), the DEMO server will be closed to external users. Please use the standalone package during this period. Jobs already submitted will not be affected. We apologize for any inconvenience this may cause.


DEMO On-line Server [View example of output for 2-domain or 3-domain protein]


DEMO Download
References:

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