If a sequence or loop is sent to TASSER for evaluation with modification of constraints for the modeling of a 212 amino acid sequence, should the DIST modification represent the sequential number of the structure from the gpcr i.e ( DIST 223 CA 436 CA 18.2) it was obtained from or simply, start the sequence with the beginning number for the segment submitted i.e (DIST 1 CA 212 CA 18.3) ???? or does it matter???
Thanks in advance,
Henry Levenson