Hello,
I was trying to use the I-TASSER V2.1 Package for large-scale protein structure prediction, but it doesn't work. here are some problems,
1. Does it only allow the query sequence named "seq.fasta"? the other file names are not allowed? such as P00533.fasta, and how many protein sequences can be contained in "seq.fasta"? only one? Could you tell me how should I do if I want to predict the structures of more than 300 proteins simultaneously.
2. When the I-TASSER (runI-TASSER.pl) was running, sometimes there were some errors for the running 'psiblast', just as follows,
[blastpgp] WARNING: [000.000] Failed to retrieve sequence gi|86147744
[blastpgp] WARNING: [000.000] Unable to open nr.00.pin
[blastpgp] WARNING: [000.000] Unable to open nr.01.pin
[blastpgp] WARNING: [000.000] Unable to open nr.00.pin
[blastpgp] WARNING: [000.000] Unable to open nr.01.pin
[blastpgp] WARNING: [000.000] Unable to open nr.pin
[blastpgp] WARNING: [000.000] Unable to open nr.pin
please tell me how to solve these problems. I think there is no problem with the Installation of I-TASSER, and the needed template library files are also installed.