When running the standalone version of ITASSER I get the following error:
FORTRAN STOP
At line 522 of file zal33.f
Fortran runtime error: End of file
Illegal division by zero at /home/ngs/nexus/itasser/scn5a/MUSTER_SCN5A_WT line 585.
The error appears at the following point of the process:
Your setting is:
-libdir =/home/ngs/itasser
-seqname =SCN5A_WT
-datadir =/home/ngs/nexus/itasser/scn5a
-usrname =lgenuser
-runstyle =serial
-idcut =1
-ntemp =20
-nmodel =5
1. make seq.txt and rmsinp
2.1 run Psi-blast
2.3 run solve
2.3 run solve
2.4 run pairmod
2.4.1 removing homology templates based on and 1
2.4.2 running pair ................
3.1 do threading
start serial threading PPA
/tmp/lgenuser/ITSCN5A_WT
/home/ngs/nexus/itasser/scn5a/PPA_SCN5A_WT
hostname: bngs02b
starting time: Sat Nov 9 21:52:54 CET 2013
pwd: /tmp/lgenuser/ITSCN5A_WT
running zalign .....
ending time: Sat Nov 9 22:13:55 CET 2013
start serial threading dPPA
/tmp/lgenuser/ITSCN5A_WT
/home/ngs/nexus/itasser/scn5a/dPPA_SCN5A_WT
hostname: bngs02b
starting time: Sat Nov 9 22:13:56 CET 2013
pwd: /tmp/lgenuser/ITSCN5A_WT
running zalign .....
ending time: Sat Nov 9 22:40:09 CET 2013
start serial threading dPPA2
/tmp/lgenuser/ITSCN5A_WT
/home/ngs/nexus/itasser/scn5a/dPPA2_SCN5A_WT
hostname: bngs02b
starting time: Sat Nov 9 22:40:11 CET 2013
pwd: /tmp/lgenuser/ITSCN5A_WT
running zalign .....
ending time: Sat Nov 9 23:06:29 CET 2013
start serial threading sPPA
/tmp/lgenuser/ITSCN5A_WT
/home/ngs/nexus/itasser/scn5a/sPPA_SCN5A_WT
hostname: bngs02b
starting time: Sat Nov 9 23:06:31 CET 2013
pwd: /tmp/lgenuser/ITSCN5A_WT
running zalign .....
ending time: Sat Nov 9 23:45:50 CET 2013
start serial threading MUSTER
/tmp/lgenuser/ITSCN5A_WT
/home/ngs/nexus/itasser/scn5a/MUSTER_SCN5A_WT
hostname: bngs02b
starting time: Sat Nov 9 23:45:52 CET 2013
pwd: /tmp/lgenuser/ITSCN5A_WT
running Psi-blast .....
running zalign .....
./zalign 7.01 0.55 0.66 1.6 -0.99 0.31 0.19 0 1.0 0.39 0.19
start serial threading wPPA
/tmp/lgenuser/ITSCN5A_WT
/home/ngs/nexus/itasser/scn5a/wPPA_SCN5A_WT
hostname: bngs02b
starting time: Sun Nov 10 00:22:58 CET 2013
pwd: /tmp/lgenuser/ITSCN5A_WT
running Psi-blast .....
ending time: Sun Nov 10 02:03:56 CET 2013
start serial threading wdPPA
/tmp/lgenuser/ITSCN5A_WT
/home/ngs/nexus/itasser/scn5a/wdPPA_SCN5A_WT
hostname: bngs02b
starting time: Sun Nov 10 02:03:57 CET 2013
pwd: /tmp/lgenuser/ITSCN5A_WT
running Psi-blast .....
ending time: Sun Nov 10 04:04:46 CET 2013
start serial threading wMUSTER
/tmp/lgenuser/ITSCN5A_WT
/home/ngs/nexus/itasser/scn5a/wMUSTER_SCN5A_WT
I already use the newest version of zal33.f (see one of the previous posts where someone had a problem with zal33). When running itasser with a smaller protein (459AA), I don't get this error. The protein I used here was 2016 AA. Could that be the cause of this error?