Hello,
I unsuccessfully run I-TASSER5.1 locally on UBUNTU app in Windows 10 and the run log is as below;
atajera@LAPTOP-2STV4UM6:/mnt/c/I-Tasser/Suite/I-TASSER5.1$ I-TASSERmod/runI-TASSER.pl -libdir /mnt/c/I-Tasser/Library -seqname PmRBP1 -datadir /mnt/c/I-Tasser/Proteins/seq.fasta
Your setting for running I-TASSER is:
-pkgdir = /mnt/c/I-Tasser/Suite/I-TASSER5.1
-libdir = /mnt/c/I-Tasser/Library
-java_home = /usr
-seqname = PmRBP1
-datadir = /mnt/c/I-Tasser/Proteins
-outdir = /mnt/c/I-Tasser/Proteins
-runstyle = serial
-homoflag = real
-idcut = 1
-ntemp = 20
-nmodel = 5
-light = false
-hours = 50
-LBS = false
-EC = false
-GO = false
1. make seq.txt and rmsinp
Your protein contains 984 residues:
> PmRBP1
MFFFKGQTRFRNIEQFFFFFFFIKNLRTTNGENVLEDIKGKDGNLDFYSLDYNSKKANKL
KYNREKKIYIKNMNFLGEKGNIRNIDNVQSEDVVVAHSSNDISFENLKGSNIGKNLSNHV
YIKKDSLYNSNENNNIKGEINNEKNYIAKSFIHNEEEAYNIRKSLYDKMDQHAVFNPFID
MEIDFIDLQYFKDILDLIPGNTSYSKYYNEEFKKIIDEYSDILSNLVKTCITEKMELIKL
EHEIKYPKKDSMEKKLEEKSIELESKRELYHNKLNNYYKNIKPKMDEVRNKGHALLQESY
CTENCSTYIAKYDDLVEKILLDIKNYGNKGHVVLEKSINDFSFLDMILQYSNQQNNDMRE
SINTLQLLGEEIKEISEIYLINSTLINDLTNFFLEIKKIKEPIDSKQFTEKLKTLIRNSC
LFRHIHKFNEPITQIYETKVTSSNKLFTSVIEKLQNETESLIKTLLDDLEFHEIRKNSEE
ITNYVKNMYDKNKELYDSMIKGLDENINLKIDKLDEFLSYQYYINDDIVNYNDFISLKYK
HIYIHLKAECEKDLRERKLLPNNVLKAKLFLEIIIKIKADEKIISESFDTAKKFYEKIKD
LKKEFEEAYVEFEEVKNEINKMKNIDDNRDKNIEEIKDKLEHITKKQNNLKEVAALKDKG
NVEITASDELLAIIPNKNENEFKNKIDKTRDDMNSLFKSLNNDNINRLIESTEEFVNQKK
NISFEDMASEVIENHLQDIRNKFDKINFISDDKMKELHDKMKEQVTIAENIKKEIVKKEI
ENLQKELAKFFSTFNSEQQELLISLEEYKREGEKIQKYRDSLLKRENEYFSSVHVDTNDL
EKTKNQAELDKLQDTFAKKKDEISRKINNIKDLINTANPHLNFYSFVEKYFNINEDKKGV
ENIKALKDKINNVDMNKQVLELENDFKRKTDALENDIISIKSITKKLISLSTLNKNINQC
DENNGAAELLKNNAKRLREEVEKE
2.1 run Psi-blast
2.2 Predict secondary structure with PSSpred...
2.3 Predict solvent accessibility...
2.4 run pairmod
2.4.1 Use all templates
2.4.2 running pair ................
FORTRAN STOP
30000 6379375 total lib str & residues
number of observations 22.62212 6.5712000E+07
pair done
3.1 do threading
start serial threading PPAS
/tmp/atajera/ITPmRBP1
/mnt/c/I-Tasser/Proteins/PPAS_PmRBP1
hostname: LAPTOP-2STV4UM6
starting time: Sun Jun 2 01:11:34 DST 2019
pwd: /tmp/atajera/ITPmRBP1
running zalign .....
ending time: Sun Jun 2 01:33:13 DST 2019
start serial threading dPPAS
/tmp/atajera/ITPmRBP1
/mnt/c/I-Tasser/Proteins/dPPAS_PmRBP1
At line 440 of file ppa1.f
Fortran runtime error: No such file or directory
Illegal division by zero at /mnt/c/I-Tasser/Proteins/dPPAS_PmRBP1 line 356.
hostname: LAPTOP-2STV4UM6
starting time: Sun Jun 2 01:33:15 DST 2019
pwd: /tmp/atajera/ITPmRBP1
running zalign .....
start serial threading dPPAS2
/tmp/atajera/ITPmRBP1
/mnt/c/I-Tasser/Proteins/dPPAS2_PmRBP1
At line 440 of file ppa1.f
Fortran runtime error: No such file or directory
Illegal division by zero at /mnt/c/I-Tasser/Proteins/dPPAS2_PmRBP1 line 355.
hostname: LAPTOP-2STV4UM6
starting time: Sun Jun 2 01:34:06 DST 2019
pwd: /tmp/atajera/ITPmRBP1
running zalign .....
start serial threading Env-PPAS
/tmp/atajera/ITPmRBP1
/mnt/c/I-Tasser/Proteins/Env-PPAS_PmRBP1
hostname: LAPTOP-2STV4UM6
starting time: Sun Jun 2 01:34:55 DST 2019
pwd: /tmp/atajera/ITPmRBP1
running zalign .....
ending time: Sun Jun 2 02:40:16 DST 2019
start serial threading MUSTER
/tmp/atajera/ITPmRBP1
/mnt/c/I-Tasser/Proteins/MUSTER_PmRBP1
At line 1067 of file zal33.f
Fortran runtime error: No such file or directory
Illegal division by zero at /mnt/c/I-Tasser/Proteins/MUSTER_PmRBP1 line 599.
hostname: LAPTOP-2STV4UM6
starting time: Sun Jun 2 02:40:19 DST 2019
pwd: /tmp/atajera/ITPmRBP1
running Psi-blast .....
running zalign .....
start serial threading wPPAS
/tmp/atajera/ITPmRBP1
/mnt/c/I-Tasser/Proteins/wPPAS_PmRBP1
Exception in thread "main" java.lang.ArrayIndexOutOfBoundsException: Index 66 out of bounds for length 66
at d.a(d.java)
at d.main(d.java)
hostname: LAPTOP-2STV4UM6
starting time: Sun Jun 2 03:06:49 DST 2019
pwd: /tmp/atajera/ITPmRBP1
running Psi-blast .....
ending time: Sun Jun 2 06:09:52 DST 2019
start serial threading wdPPAS
/tmp/atajera/ITPmRBP1
/mnt/c/I-Tasser/Proteins/wdPPAS_PmRBP1
Exception in thread "main" java.io.FileNotFoundException: /mnt/c/I-Tasser/Library/DEP/1a4zA.dep (No such file or directory)
at java.base/java.io.FileInputStream.open0(Native Method)
at java.base/java.io.FileInputStream.open(FileInputStream.java:219)
at java.base/java.io.FileInputStream.(FileInputStream.java:157)
at java.base/java.io.FileInputStream.(FileInputStream.java:112)
at java.base/java.io.FileReader.(FileReader.java:60)
at c.a(c.java)
at c.main(c.java)
Illegal division by zero at /mnt/c/I-Tasser/Proteins/wdPPAS_PmRBP1 line 348.
hostname: LAPTOP-2STV4UM6
starting time: Sun Jun 2 06:09:55 DST 2019
pwd: /tmp/atajera/ITPmRBP1
running Psi-blast .....
start serial threading wMUSTER
/tmp/atajera/ITPmRBP1
/mnt/c/I-Tasser/Proteins/wMUSTER_PmRBP1
Exception in thread "main" java.lang.ArrayIndexOutOfBoundsException: Index 75 out of bounds for length 75
at e.main(e.java)
Illegal division by zero at /mnt/c/I-Tasser/Proteins/wMUSTER_PmRBP1 line 570.
hostname: LAPTOP-2STV4UM6
starting time: Sun Jun 2 11:29:04 DST 2019
pwd: /tmp/atajera/ITPmRBP1
running Psi-blast .....
running Psi-blast .....
only 3 threading programs have output, please check threading programs
atajera@LAPTOP-2STV4UM6:/mnt/c/I-Tasser/Suite/I-TASSER5.1$
I would appreciate any help to direct me to solve the problem.