The following total binding energy values were generated for a protein complex using EvoEF2 BuildMutant and then ComputeBinding. One mutation was applied for each run. These are partial list of all different combinations, such as L351A, L351D, etc.
L351L -52.27
G352G -52.27
K353K -55.31
G354G -52.27
D355D -52.15
F356F -52.15
R357R -51.66
My question is why some of the values are different than others while these mutations make no change to the protein?