(The server completed predictions for 202690 proteins submitted by 50756 users from 116 countries)
(The template library was updated on 2014/11/26)

I-TASSER server is an on-line platform for protein structure and function predictions. 3D models are built based on multiple-threading alignments by LOMETS and iterative template fragment assembly simulations; function inslights are derived by matching the 3D models with BioLiP protein function database. I-TASSER (as 'Zhang-Server') was ranked as the No 1 server for protein structure prediction in recent CASP7, CASP8, CASP9, and CASP10 experiments. It was also ranked as the best for function prediction in CASP9. The server is in active development with the goal to provide the most accurate structural and function predictions using state-of-the-art algorithms. The server is only for non-commercial use. Please report problems and questions at I-TASSER message board and some members will study and answer the questions asap. (>> More about the server ...)

Download I-TASSER Standalone Package (Version 4.2)

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Copy and paste your sequence here (<1,500 residues, in FASTA format):

Or upload the sequence from your local computer:

Email: (mandatory, where results will be sent to)

Password: (mandatory, please click here if you do not have a password)

ID: (optional, your given name of the protein)

Option I: Assign additional restraints & templates to guide I-TASSER modeling.

  • Assign contact/distance restraints
  • Atom-atom restraints Explanation
  • Specify template without alignment
  • Type a PDB ID Explanation
  • Specify template without alignment
  • Upload a structure Explanation
  • Specify template with alignment
  • Template & alignment Explanation

Option II: Exclude some templates from I-TASSER template library.

(Please submit a new job only after your old job is completed)

  • 2014/10/27: I-TASSER V4.2 was released! Read Whatisnew4.2.txt to check the new features added to I-TASSER V4.2 compared to the previous versions.
  • 2014/08/14: I-TASSER V4.1 was released! Read Whatisnew4.1.txt to check the new features added to I-TASSER V4.1 compared to the previous versions.
  • 2014/06/18: I-TASSER V4.0 was released! Read Whatisnew4.0.txt to check the new features added to I-TASSER V4.0 compared to the previous versions.
  • 2014/06/09: New development on residue-specific local-structure accuracy and B-factor estimations was included in the I-TASSER pipeline. This new feature allows users to track the error and fluctuation of each residue in the I-TASSER modeling and make a better use of the I-TASSER models.
  • 2014/01/24: I-TASSER V3.0 is released! Read Whatisnew3.0.txt to check the new features added to I-TASSER V3.0 compared to the previous versions.

    >> Read more I-TASSER news ...

  • Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, vol 9, 40 (2008). (download the PDF file).
  • Ambrish Roy, Alper Kucukural, Yang Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, vol 5, 725-738 (2010). (download the PDF file).
  • Ambrish Roy, Jianyi Yang, Yang Zhang. COFACTOR: An accurate comparative algorithm for structure-based protein function annotation. Nucleic Acids Research, 40: W471-W477 (2012). (download the PDF file).

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