(The server completed predictions for 142378 proteins submitted by 37451 users from 113 countries)
(The template library was updated on 2013/06/14)

I-TASSER server is an on-line platform for protein structure and function predictions. 3D models are built based on multiple-threading alignments by LOMETS and iterative template fragment assembly simulations; function inslights are derived by matching the 3D models with BioLiP protein function database. I-TASSER (as 'Zhang-Server') was ranked as the No 1 server for protein structure prediction in recent CASP7, CASP8, CASP9, and CASP10 experiments. It was also ranked as the best for function prediction in CASP9. The server is in active development with the goal to provide the most accurate structural and function predictions using state-of-the-art algorithms. The server is only for non-commercial use. Please report any problems and questions at I-TASSER message board and some members will study and answer the questions asap. (>> More about the server ...)

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Copy and paste your sequence here (<1,500 residues, in FASTA format): Or upload the sequence from your local computer: Email: (mandatory, where results will be sent to) Password: (mandatory, please click here if you do not have a password) ID: (optional, your given name of the protein) Option I: Assign additional restraints & templates to guide I-TASSER modeling. Assign contact/distance restraints Atom-atom restraints Explanation Specify template without alignment Type a PDB ID Explanation Specify template without alignment Upload a structure Explanation Specify template with alignment Template & alignment Explanation (Read more explanation on how to add restraints) Assign contact/distance restraints Atom-atom restraints Explanation Specify template without alignment Type a PDB ID Explanation Specify template without alignment Upload a structure Explanation Specify template with alignment Template & alignment Explanation Option II: Exclude some templates from I-TASSER template library. Exclude homologous templates Type a cutoff (e.g. '60%') Explanation Exclude specific template proteins Upload a file listing all PDB IDs Explanation (Please submit a new job only after your old job is completed)

• 2013/5/04: A bug of incomplete array transfer in the I-TASSER program got fixed.
• 2013/4/09: A new strategy was introduced to select the first models based on consensus with the threading alignments. This stretagy increases the accuracy of the first models by ~40% for the targets which have the first models inconsistent with threading.
• 2013/2/25: A link was added at each output page to allow users to download all modeling results in a tarball file.
• 2013/2/14: I-TASSER Server updated the strategy for modeling big proteins. This strategy fixes a problem that minority templates with a longer alignment length may dominate the assembly simulations.
• 2012/10/23: I-TASSER V2.1 is released with bugs in former versions fixed (check details in Whatisnew2.1.txt).

  >> Read more I-TASSER news ...

• Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, vol 9, 40 (2008). (download the PDF file).
• Ambrish Roy, Alper Kucukural, Yang Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, vol 5, 725-738 (2010). (download the PDF file).
  
• Ambrish Roy, Jianyi Yang, Yang Zhang. COFACTOR: An accurate comparative algorithm for structure-based protein function annotation. Nucleic Acids Research, 40: W471-W477 (2012). (download the PDF file).