Hi,
I'm running a simulation with either the SPRING or COTH with a ~300 residue protein, but the server responded an error "first sequence contains non standard residues. Please make sure your sequence contains only the 20 standard residues".
Then I tried to run a test simulation with the residues from the example of the SPRING output, but the same error still occurring.
Thank you in advance for any help.
Amanda
### My sequences
>Em_A
MSEPVKVCVTGAAGQIAYSLLYSIAKGDVFGSDQPIILTLLDIAPMEGVLNGVVMELMDCAMPLLKEVIPTCDEKVAFKDIDAAFLVGAMPRREGMERKDLLAANIKIFKSQGGALDTVAKKTVKVVVVGNPANTNALICSKYAPSIPKENFSCLTRLDQNRAQAQIAARLGIPNSAVSNVIIWGNHSSTQFPDVRHAKAIVNGKTVPVPEAVKDDNYLKNEFLTTVQKRGGEVIKARKLSSAMSAAKATADHMRDWWFGTQGDNWVSMGVFSTGAYGIPEGIMYSYPVRIANKQWKIVEGLEINDFAREKMDLTAKELCEERAMAEEICNQ
>Em_B
MSEPVKVCVTGAAGQIAYSLLYSIAKGDVFGSDQPIILTLLDIAPMEGVLNGVVMELMDCAMPLLKEVIPTCDEKVAFKDIDAAFLVGAMPRREGMERKDLLAANIKIFKSQGGALDTVAKKTVKVVVVGNPANTNALICSKYAPSIPKENFSCLTRLDQNRAQAQIAARLGIPNSAVSNVIIWGNHSSTQFPDVRHAKAIVNGKTVPVPEAVKDDNYLKNEFLTTVQKRGGEVIKARKLSSAMSAAKATADHMRDWWFGTQGDNWVSMGVFSTGAYGIPEGIMYSYPVRIANKQWKIVEGLEINDFAREKMDLTAKELCEERAMAEEICNQ
### Sequences from the example
>Sequence A
PYTVVYFPVRGRCAALRMLLADQGQSWKEEVVTVETWQEGSLKASCLYGQLPKFQDGDLT
LYQSNTILRHLGRTLGLYGKDQQEAALVDMVNDGVEDLRCKYISLIYTNYEAGKDDYVKA
LPGQLKPFETLLSQNQGGKTFIVGDQISFADYNLLDLLLIHEVLAPGCLDAFPLLSAYVG
RLSARPKLKAFLASPEYVNLPINGNGKQ
>Sequence B
PYTVVYFPVRGRCAALRMLLADQGQSWKEEVVTVETWQEGSLKASCLYGQLPKFQDGDLT
LYQSNTILRHLGRTLGLYGKDQQEAALVDMVNDGVEDLRCKYISLIYTNYEAGKDDYVKA
LPGQLKPFETLLSQNQGGKTFIVGDQISFADYNLLDLLLIHEVLAPGCLDAFPLLSAYVG
RLSARPKLKAFLASPEYVNLPINGNGKQ