I would like to build a homology model of my enzyme using I-TASSER, however because the side directed mutagenesis are not possible in this enzyme at the moment, we are trying to chemically modify the active site amino acids (by e.g. acetylation of Tyr). To further validate the results of our kinetic and spectroscopic experiments we would like to build a homology model of MCR with solvent accessible tyrosines being acetylaled. Would it be possible with I-TASSER and if so how do we do that? How do I name aa in a sequence so it will be recognized by the server as acetylated?
Thank you.
Best regards,
dsliwa