I appreciate your attention, I am learning how a variant could produce an effect on a protein and I found myself with iTASSER and STRUM, I entered the WT sequence in iTASSER and I got the following,
https://zhanglab.ccmb.med.umich.edu/I-TASSER/output/S444322/
and I introduced the MUT sequence and got this other,
https://zhanglab.ccmb.med.umich.edu/I-TASSER/output/S444558/
between both I do not observe important difference so I think there was no major change, when I introduce the sequence whit a variant to STRUM I have the following,
http://zhanglab.ccmb.med.umich.edu/STRUM/output/SM103786
what I see is a protein that at one point is denatured with a ddG of -0.96 that according to an article of STRUM indicates that the variant causes destabilization, this is correct?, this means that because of the variant we have a temperature-sensitive protein at the point of change or termolabil effect?, and if it had a variant with ddG of -1.41 in comparison with -0.96 only means that -1.41 is more sensitive to temperature changes than -0.96?, I will appreciate your guide and if there is something that I am not considering I would like to let me know